Zogulitsa
Trimethylol aminomethane
Gwiritsani ntchito
① Pakati pa fosfomycin, itha kugwiritsidwanso ntchito ngati vulcanization accelerator, zodzoladzola (kirimu, mafuta odzola), mafuta amchere, parafini emulsifier, biological buffer.
② Amagwiritsidwa ntchito ngati kuyamwa gasi wa asidi, kukonzekera kwa buffer, surfactant, emulsifier ndi accelerator.Amagwiritsidwanso ntchito mu organic synthes.
Njira Yopangira
Sungunulani Tris ndi madzi pang'ono owirikiza kawiri (300-500ml), onjezani HCl, sinthani pH kufika pa 7.6 ndi HCl (1N) kapena NaOH (1N), ndipo potsirizira pake yikani madzi owirikiza kawiri ku 1000ml.Madzi awa ndi madzi osungira, osungidwa mufiriji pa 4 ℃.
Zindikirani: Mtengo wa PH wa Tris-Hcl umasiyana ndi kutentha, kotero uyenera kuyeza kutentha kwa chipinda, kotero zotsatira zoyezedwa ndizodalirika.
Chikhalidwe cha buffering
Tris ndi maziko ofooka omwe ali ndi pKa ya 8.1 kutentha (25 ℃).Malinga ndi chiphunzitso cha buffering, ma buffering osiyanasiyana a Tris buffers ali pakati pa pH7.0 ndi 9.2.
PH yamadzimadzi amadzimadzi a Tris base ndi pafupifupi 10.5, ndipo chotchinga cha pH mtengo chikhoza kupezeka powonjezera hydrochloric acid kuti musinthe mtengo wa pH pamtengo womwe mukufuna.Komabe, chidwi chiyenera kuperekedwa ku mphamvu ya kutentha pa pKa ya Tris.Digiri, PH mtengo watsika ndi 0.03.
1M Tris-HCl 6.8 ndi 1.5M Tris-HCl 8.8 ndizomwe zimagwiritsidwa ntchito kwambiri pa SDS-PAGE.
Ngakhale kuti TAE, TBE ndi ma reagents ena opangidwa kuchokera ku Tris ndi omwe amagwiritsidwa ntchito kwambiri pa DNA electrophoresis, TE (pH8.0) amagwiritsidwa ntchito makamaka pakusungunuka kwa DNA.(TE ndi Tris kuphatikiza EDTA.)
Tris buffers samangogwiritsidwa ntchito kwambiri ngati zosungunulira za nucleic acid ndi mapuloteni, komanso amakhala ndi ntchito zambiri zofunika.Tris imagwiritsidwa ntchito pakukula kwa protein crystal pansi pazikhalidwe zosiyanasiyana za pH.Mphamvu yotsika ya ionic ya Tris buffer ingagwiritsidwe ntchito popanga ulusi wapakati wa lamin mu C. elegans.Tris ndi chimodzi mwa zigawo zikuluzikulu za mapuloteni electrophoresis buffers.Kuphatikiza apo, Tris ndi wapakatikati pokonzekera ma surfactants, ma vulcanization accelerators, ndi mankhwala ena.Tris imagwiritsidwanso ntchito ngati mulingo wa titration.
Mu mawonekedwe azithunzi ndi zolemba, zitha kuphatikiza zambiri zamalonda, mautumiki ndi maubwino.Itha kupitilira malondawo kuti muwonjezere zina (zodetsa nkhawa zamakasitomala, nkhawa, ndi zina), kuteteza zachilengedwe, zitsanzo zaulere, ndi zina zambiri.
1. Zitsanzo zimayesedwa kwaulere kuti zitsimikizire kuti makasitomala amagwiritsa ntchito bwino.
2. Malingana ndi zofuna za makasitomala osiyanasiyana, ang'onoang'ono mpaka 100g, aakulu mpaka matani a migolo, akhoza kukwaniritsa zofunikira za phukusi.
3. Malipiro osinthika, kusamutsa pa telegraph kapena kuvomereza (kukwaniritsa zofunikira pakulandila)
4. Kuthamanga kwachangu, tsiku lomwelo kapena tsiku lotsatira likhoza kuperekedwa, ndondomeko yonse yotsatila chidziwitso cha kayendetsedwe ka kayendetsedwe kake, kuonetsetsa kuti makasitomala akugwiritsidwa ntchito panthawi yake.
5. Utumiki wabwino kwambiri pambuyo pa malonda, oleza mtima komanso osamala kuthetsa mitundu yonse ya mavuto omwe angachitike, monga mavuto a khalidwe, kugwirizana kwathunthu ndi makasitomala kuti athane nawo mwakhama, musazengereze udindo ndi kuyankha mosasamala.
6. Gulu labwino kwambiri, luso logwira ntchito bwino komanso chidziwitso cha akatswiri zimapangitsa makasitomala kukhala otsimikiza za mankhwala athu, komanso amamva kuti gulu lathu ndi lodalirika.
7. Ufulu wodziyimira pawokha wotumiza kunja kumanga msika wapadziko lonse lapansi komanso chikoka chamtundu wapadziko lonse lapansi.
8. Woona mtima, kalata yochokera, zaka 20 za mbiri ya kampani ndi mbiri yabwino, lolani makasitomala kukhala omasuka, kupindula ndi kupambana-kupambana, mgwirizano woona mtima.
